Protein ligand interactions methods and applications second edition provides a complete introduction to common and emerging procedures for characterizing the interactions of individual proteins from the initial discovery of natural substrates or potential drug leads to the detailed quantitative understanding of the mechanism of interaction all stages of the research process are covered with a focus on those techniques that are or are anticipated to become widely accessible and . Protein ligand interactions methods and applications second edition provides a complete introduction to common and emerging procedures for characterizing the interactions of individual proteins. Authoritative and accessible protein ligand interactions methods and applications second edition serves as an ideal guide for researchers new to the field of biophysical characterization of protein interactions whether they are beginning graduate students or experts in allied areas of molecular cell biology microbiology pharmacology medicinal chemistry or structural biology. The protocols follow the successful methods in molecular biology series format each offering step by step laboratory instructions an introduction outlining the principle behind the technique lists of the necessary equipment and reagents and tips on troubleshooting and avoiding known pitfalls